بررسي ميزان بيان ژن هاي مرتبط با تكثير سلولي در رده سلولي كارسينوم سلول سنگفرشي انساني (A431) ، به دنبال كشت همزمان آن با سلول هاي بنيادي مزانشيمي مشتق از بافت چربي انساني (ASC)

Introduction: Skin cancer is the most common human cancer in the world. Squamous cell carcinoma (SCC) is the second most common skin malignancy derived from the epidermis, and its preva‎lence has increased worldwide. During recent studies, the incidence of skin cancer in Gilan province has also been on the rise and has been increasing in different age groups. A wide range of treatment options are available to treat this type of cancer. One of these new methods in this study is the use of stem cells derived from fat tissue. Therefore, the aim of this study is to investigate the effect of adipose tissue stem cell (ASC) supernatant on the viability of A431 squamous cell carcinoma cell line. In addition, considering the role of two important cell signaling pathways (JAK2-STAT3 and PI3K/AKT/mTOR) in the growth and proliferation of cancer, in this study, the effect of ASC culture medium on the gene expression of these two pathways (JAK2-STAT3 and PI3K/AKT/mTOR) were investigated. Materials and Methods: In order to conduct this study, A431 cell line was co-cultured with ASCs in two-layer 6-well plate (microplate insert system) for 96 hours. Finally, MTT assay was used to check the viability of A431 cell line in the test group and compare it with the control group. Real-time PCR technique was used to investigate the rates of expression changes of JAK2, STAT3, mTOR and PI3K genes in the test group compared to the control group, after 96 hours of co-culture of A431 cell line with ASCs. Results: During this study, the viability of A431 cell line increased compared to the control group. Nevertheless, the results of gene expression analysis showed that the expression level of all 4 genes in the test group has significantly decreased compared to the control group. Conclusion: Considering that the viability of A431 cell line increased in co-culture with ASCs, compared to the control group, and led to increased proliferation of this cell line, and on the other hand, the expression level of genes involved in cell proliferation decreased, It can be concluded that the investigated signaling pathways were not part of the main effective pathways in the proliferation of this cancer cell line, or depending on the type of cancer cells, different signaling pathways were involved, which requires more studies.

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